Microscope Camera: Illumination Types & Filters

Published on August 21, 2016

Originally published in Mikroskopie in January 2016, this article was written by J. Piper and M. Torzewski. The English translation, written by Amy Groth, was serialized into: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 and 11.

Illumination Types and Filters

In addition to bright-field images, images produced via polarized light, darkfield and phase contrast techniques can be flawlessly visualized, photographed and filmed. Even very narrowly filtered monochromatic light (red, green and blue) did not pose any problems for the camera-neither with regard to the exposure nor the precision of the autofocus. Monochromatically filtered objects produced flawless black-and-white images. Figs. 13, 14, 15 and 16 show some application examples which were taken using various types of lighting, filters and microscopes.

Fig. 13. Image from camera with Meiji eyepiece on a 30 mm large-field tube von Carl Zeiss Jena in combination with a plan-corrected Leitz objective NPL 40/0.65, rat embryo, cartilagenous vertebral column, bright-field, usable result despite missing compensating eyepiece.

Fig. 14. Camera with Meiji eyepiece a 30 mm large-field tube von Carl Zeiss Jena, Leitz plan-corrected scanning objective Pl Fl 4/0.15, alum crystal without cover slip, darkfield, unfiltered halogen light (top), 'minusviolet' type filter (Baader Semi-Apo-Filter, middle), monochromatic green filter 540 nm (Baader Solar-Continuum-Filter, below). Focus with One-Push autofocus.

Fig. 15. Camera with Meiji eyepiece a 30 mm large-field tube von Carl Zeiss Jena, Leitz plan-corrected scanning objective Pl 2.5/0.8, xylit crystal without cover slip, polarization, lambda compensator, One Push autofocus.

Fig. 16. Camera with Meiji eyepiece a 30 mm large-field tube von Carl Zeiss Jena, Leitz plan-corrected scanning objective Pl Fl 4/0.15, xylit crystal without cover slip, polarization, without compensator, One Push autofocus.

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Post published by TIS Marketing on August 21, 2016.

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